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The velocity is not necessarily the same at all times after you start the reaction The depletion of substrate, inhibition by the product, or instability of the enzyme can cause the velocity to change with time The initial velocity is measured early, before the velocity changes Initial velocity measurements also let you assume that the amount of substrate has not changed and is equal to the amount of substrate that was added

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close to what you started with It s obviously changed (or you couldn t have measured it), but it s not changed all that much When you stick with the initial part of the velocity measurement, the velocity is less likely to change due to substrate depletion, or product inhibition, and you re more likely to know what the actual substrate concentration is Initial velocity measurements help avoid the dreaded curvature

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HAVE THE TIME TO WASTE It is possible to decide that something is just not worth remembering; for example, cleavage specificities of proteases or restriction endonucleases, and protein molecular weights, are

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