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(As with the northern-hemispheric situation, there is some variance in the Moon s tilt Depending on the season of the year, moonrise and moonset occur somewhat north or south of due east or west) The waxing crescent is in the northwestern or western sky just after sunset and sets 2 to 4 hours after the Sun The Moon at first quarter is in the northern sky at sunset, moves generally westward, and sets around midnight The waxing gibbous Moon is in the northeast at sunset, moves to the west, and sets a couple of hours before dawn The full Moon rises around sunset and sets around sunrise, tracking across the northern half of the sky during the night The waning gibbous Moon rises shortly after sunset and sets after sunrise the next day The last-quarter Moon rises at about midnight and sets around noon The waning crescent rises a couple of hours before dawn and sets in the afternoon

Figure 8-1

With hand facing backward and first two fingers up, place hand at side of head and wiggle fingers 132 | Baby Signs

The VELOCITY of product formation (or substrate disappearance) is defined as the change in product concentration per unit time It is the slope of a plot of product concentration against time The velocity of product formation is the same as the velocity of substrate disappearance (except that substrate goes away, whereas product is formed)

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Swing fingertips of V hand rhythmically back and forth over open palm Baby Signs Dictionary | 137

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canceled out, and you don t see it because both the product and enzyme are expressed in concentration units; both are per milliliter Let s do a numerical example Adding 01 g of fumarase to a solution of 5 mM fumarate (substrate) in a final volume of 10 mL results in the formation of 0024 mol of malate (product) per minute At the beginning of the reaction, the concentration of product is zero If we wait 10 min 10 0024 mol of malate will be made, or 024 mol In a volume of 1 mL, 024 mol represents a concentration of 024 mM So, over a period of 10 min, the concentration of malate went from 0 to 024 mM while the concentration of fumerate went from 5 to 476 mM (5 mM 024 mM) The specific activity of the enzyme would be [0024 mol/(min mL)]/(01 10 3 mg fumarase/mL) or 240 mol/(min mg) To make matters worse, velocity is often reporting using the change in the amount of product per time ( mol/min) To actually determine the concentration, you need to know the volume The key unit that always shows up somewhere with velocities and never cancels out is the per time part; the rest can usually be sorted out, depending on whether you re dealing with amounts or concentrations

Squeeze both fists together, then open and close them as you move horizontally outward 146 | Baby Signs

This is the measurement of the rate under conditions under which there is no significant change in the concentration of substrate As substrate is consumed, the substrate concentration falls and the reaction may get slower As product is made, the reaction may slow down if the product is an inhibitor of the enzyme Some enzymes are unstable and die as you re assaying them All these things may cause the velocity to change with time If the velocity is constant with time, the plot of product against time is a straight line; however, if velocity changes with time (the slope changes with time), this plot is curved (Fig 8-2) Usually enzyme activities are measured under conditions under which only a tiny bit of the substrate is converted to product (like 1 to 5 percent)3 This means that the actual concentration of substrate will be very

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