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The concentrations of substrate and product are invariably in molar units (M; this includes mM, M, etc), but enzyme concentrations may be given in molar (M), milligrams per milliliter (mg/mL), or units/mL The amount of enzyme you have can be expressed in molecules, milligrams, nanomoles (nmol), or units A unit of enzyme is the amount of enzyme that will catalyze the formation of 1 mol of product per minute under specifically defined conditions A unit is an amount, not a concentration Units of enzyme can be converted to milligrams of enzyme if you know a conversion factor called the specific activity Specific activity is the amount of enzyme activity per milligram of protein (micromoles of product formed per minute per milligram of protein, or units per milligram) For a given pure enzyme under a defined set of conditions, the specific activity is a constant; however, different enzymes have different specific activities To convert units of enzyme to milligrams of enzyme, divide by the specific activity: units/(units/milligram) milligrams Specific activity is often used as a rough criterion of purity, since in crude mixtures very few of the milligrams of protein will actually be the enzyme of interest There may be a large number of units of activity, but there will also be a large amount of protein, most of which is not the enzyme As the enzyme becomes more pure, you ll get the same units but with less protein, and the specific activity will increase When the protein is pure, the specific activity will reach a constant value Enzyme concentrations in milligrams per milliliter can be converted to molar units by dividing by the molecular weight (in mg/mmol)2 mg/mL mg/mmol mmol mL M

waits until the predawn hours to rise Moonset in the waning phases takes place in the daytime, and some people say that there is no moonset during this half of the lunar cycle

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The active site is the special place, cavity, crevice, chasm, cleft, or hole that binds and then magically transforms the substrate to the product The kinetic behavior of enzymes is a direct consequence of the protein s having a limited number (often 1) of specific active sites

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Most of enzyme kinetics (and mechanism) revolves around the active site As we ll see later, saturation kinetics is one of the direct consequences of an active site

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Most people envision the Moon s phase-to-phase progress and appearance as seen from the northern hemisphere This is natural because there are more people living north of the equator than south of it As far as Earth itself is concerned, however, this is only half the story Figure 4-4 shows the way the Moon looks at various stages in its orbit around Earth as seen from a midlatitude northern location such as Kansas City, Missouri, or Rome, Italy (There is some variance in the tilt, depending on the season of the year; moonrise and moonset occur somewhat north

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An assay is the act of measuring how fast a given (or unknown) amount of enzyme will convert substrate to product the act of measuring a velocity

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